KMID : 0829320070100010014
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Korean Journal of Clinical Microbiology 2007 Volume.10 No. 1 p.14 ~ p.18
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TT Virus Detection Using Different PCR Primer Sets in Healthy and Infected Individuals with Hepatitis B or C Viruses
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Kim Han-Sung
Kim Jae-Seok Song Won-Keun Kang Hee-Jung Lee Kyu-Man
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Abstract
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Background: TT virus (TTV) infection is highly prevalent in the general population and in the patients infected
with hepatitis B virus (HBV) or hepatitis C vius (HCV). The aim of the present study was to assess the positive rates of TTV DNA using different PCR primer sets in healthy and HBV or HCV-infected individuals in Korea.
Methods: TTV DNA was investigated in serum samples of 69 healthy individuals and 59 HBV-infected and 34 HCV-infected individuals by nested PCR assays using primers from N22 region, 5¡¯-untranslated region (UTR), and 3¡¯ UTR of viral genome.
Results: TTV DNA was detected in 43% of total study populations using N22 primers, in 69% using 5¡¯ UTR primers and, in 64% using 3¡¯ UTR primers. No significant difference was observed in the positive rates of TTV DNA between healthy and HBV or HCV- infected individuals.
Conclusion: The PCR assays for TTV DNA using 5¡¯ UTR primers and 3¡¯ UTR primers exhibited higher positive rates than that of the assay using N22 primers without any significant difference between healthy and HBV or HCV-infected individuals. (Korean J Clin Microbiol 2007;10:14-18)
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KEYWORD
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TT virus (TTV), Healthy individuals, Hepatitis B vius (HBV), Hepatitis C vius (HCV), Primers
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